ABSTRACT
Objective: To evaluate the anti-Candida effect of eugenol and its antimicrobial interaction with nystatin. Material and Methods: The antimicrobial potential was assessed by microdilution technique (M27A3 reference method), by determining the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) against C. albicans (ATCC 90028). The possible action of eugenol on the fungal cell wall was evaluated with the assistance of the osmotic protector sorbitol (0.8 M). The antimicrobial interaction with nystatin was assessed through the checkerboard method. All tests were performed in triplicate. Results: All groups showed reductions in PI and GBI values and improvements in oral health knowledge, but IG1 and IG2 showed statistically significant differences in these variables compared to CG. Conclusion: The eugenol has antifungal activity against C. albicans and its mechanism of action is probably not related to damage to the fungal cell wall. Association between eugenol and nystatin was not found to be an advantageous possibility for growth inhibition of C. albican.
Subject(s)
Anti-Bacterial Agents , Candida albicans , Drug Synergism , Eugenol , Brazil , In Vitro TechniquesABSTRACT
Objetivo: Avaliar in vitro a atividade antimicrobiana docitronelol, linalol, timol e D-limoneno frente a Streptococcus mutans, S. salivarius e S. oralis. Materiale Métodos: Realizou-se um screening pela técnica de difusão em meio sólido. Discos de papel absorvente foram em bebidos com 30 μL da substância-teste e dispostos em placas dePetri contendo Ágar Müeller Hinton previamente semeadas com inóculo bacteriano. Após incubação por 24h a 37ºC, foi realizada a mensuração dos halos de inibição do crescimento bacteriano com auxílio de um paquímetro manual. A clorexidina2% foi utilizada como controle positivo. O estudo foi realizado em triplicata. Resultados: O citronelol obteve média do diâmetro dos halos, em milímetros (mm), para S. mutans, S.oralis e S. salivarius, respectivamente: 10.7, 10.0 e 9.8. Otimol, para a mesma sequência de microorganismos: 7.3, 7.3e 8.3 mm. O D-limoneno exibiu médias de 8.0, 6.7 e 6.0 mmpara S. mutans, S. oralis e S. salivarius, respectivamente.Mensurou-se médias dos halos de 8.0, 9.0 e 8.2 mm, para os mesmos microorganismos, quando testado o linalol. Aclorexidina, por sua vez, apresentou média dos diâmetros dos halos de 17 mm para S. mutans, 16.8 mm para S. oralis e19.7 mm para S. salivarius. Conclusão: Os produtos avaliados apresentaram atividade antibacteriana,representando possíveis substâncias com aplicabilidade na prevenção da cárie dentária.
Objective: To evaluate the in vitro antibacterial activity ofcitronellol, linalool, thymol, and D-limonene againstStreptococcus mutans, S. salivarius and S. oralis. Materialand Methods: It was performed a screening by the diffusiontechnique on solid medium. Absorbent paper discs weresoaked with 30 μl of the tested substance and then placedon petri dishes containing Mueller Hinton agar previously sownwith the bacterial inoculum. After incubation for 24 h at 37 °C,it was measured the halo of growth inhibition with the aid ofa manual caliper. Chlorhexidine (2%) was used as positivecontrol. Tests were performed in triplicate. Results: Forcitronellol, the means of the diameters of the halos, in millimeters (mm), were 10.7, 10.0, and 9.8 against S. mutans,S. salivarius and S. oralis respectively. The values foundfor thymol were 7.3, 7.3 and 8.3 mm, respectively. Thephytochemical D-limonene exhibited means of 8.0, 6.7 and6.0 mm, respectively. For linalool, the means of the haloswere 8.0 mm, 9.0 mm and 8.2 mm. Chlorhexidine was foundto show means of 17, 16.8 and 19.7 mm on S. mutans, S.oralis and S. salivarius respectively. Conclusion: The products under test showed antibacterial activity, being therefore potential substances to be applied for dental caries prevention.